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p akt2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p akt2
    The TIM3/GAL9 autocrine stimulatory loop is involved in ALL. (A) GAL9 expression was upregulated in both ALL bone marrow samples and ALL cell lines. (B) TARGET data showed that <t>AKT2,</t> ERK, and NF-κB were activated in ALL. (C) Representative Western blots of total AKT2, total ERK, NF-κB, and GAPDH in Jurkat and Nalm-6 cells transduced with shNC, shLnc-TIM3#1, or shLnc-TIM3#2. Data were shown as mean ± SD and one-way ANOVA was used for multiple-group comparison. **, P<0.01; ****, P<0.0001. ALL, acute lymphoblastic leukemia; ANOVA, analysis of variance; NC, negative-control; SD, standard deviation.
    P Akt2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+akt2/Phospho-Akt2+(Ser474)+Rabbit+mAb/pmc13657841-97-7-10
    Average 95 stars, based on 189 article reviews
    p akt2 - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Lnc-TIM3 promotes acute lymphoblastic leukemia progression through miR-5094-mediated regulation of TIM3"

    Article Title: Lnc-TIM3 promotes acute lymphoblastic leukemia progression through miR-5094-mediated regulation of TIM3

    Journal: Translational Cancer Research

    doi: 10.21037/tcr-2026-1285

    The TIM3/GAL9 autocrine stimulatory loop is involved in ALL. (A) GAL9 expression was upregulated in both ALL bone marrow samples and ALL cell lines. (B) TARGET data showed that AKT2, ERK, and NF-κB were activated in ALL. (C) Representative Western blots of total AKT2, total ERK, NF-κB, and GAPDH in Jurkat and Nalm-6 cells transduced with shNC, shLnc-TIM3#1, or shLnc-TIM3#2. Data were shown as mean ± SD and one-way ANOVA was used for multiple-group comparison. **, P<0.01; ****, P<0.0001. ALL, acute lymphoblastic leukemia; ANOVA, analysis of variance; NC, negative-control; SD, standard deviation.
    Figure Legend Snippet: The TIM3/GAL9 autocrine stimulatory loop is involved in ALL. (A) GAL9 expression was upregulated in both ALL bone marrow samples and ALL cell lines. (B) TARGET data showed that AKT2, ERK, and NF-κB were activated in ALL. (C) Representative Western blots of total AKT2, total ERK, NF-κB, and GAPDH in Jurkat and Nalm-6 cells transduced with shNC, shLnc-TIM3#1, or shLnc-TIM3#2. Data were shown as mean ± SD and one-way ANOVA was used for multiple-group comparison. **, P<0.01; ****, P<0.0001. ALL, acute lymphoblastic leukemia; ANOVA, analysis of variance; NC, negative-control; SD, standard deviation.

    Techniques Used: Expressing, Western Blot, Transduction, Comparison, Analysis, Negative Control, Standard Deviation

    Related Articles

    Incubation:

    Article Title: Nr4a1 regulates microglia overactivation-mediated neuroinflammation to ameliorate cognitive impairment in trigeminal neuralgia rats
    Article Snippet: BV2 and HT22 cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% Triton X-100 (P0096; Beyotime). .. Cells were blocked with 2% bovine serum albumin and incubated overnight at 4 °C with primary antibodies against Iba1 (1:300; ab283319, Abcam), p-Akt2 (1:200; 8599, Cell Signaling Technology), or Postsynaptic density protein 95 (PSD95) (1:200; 3409, Cell Signaling Technology). .. Subsequently, cells were incubated in the dark for 1 h with fluorescent secondary antibodies Alexa Fluor ® 555 goat anti-mouse IgG (1:200; ab150118, Abcam) or Alexa Fluor ® 647 goat anti-rabbit IgG (1:200; ab150079, Abcam), followed by staining with 4’,6-diamidino-2-phenylindole.

    Article Title: Nr4a1 regulates microglia overactivation-mediated neuroinflammation to ameliorate cognitive impairment in trigeminal neuralgia rats.
    Article Snippet: BV2 and HT22 cells were fixed with 4% paraformaldehyde 217 and permeabilized with 0.5% Triton X-100 (P0096; Beyotime). .. Cells were blocked with 2% 218 bovine serum albumin and incubated overnight at 4°C with primary antibodies against Iba1 219 (1:300; ab283319, Abcam), p-Akt2 (1:200; 8599, Cell Signaling Technology), or Postsynaptic 220 density protein 95 (PSD95) (1:200; 3409, Cell Signaling Technology). .. Subsequently, cells 221 were incubated in the dark for 1 h with fluorescent secondary antibodies Alexa Fluor® 555 222 goat anti-mouse IgG (1:200; ab150118, Abcam) or Alexa Fluor® 647 goat anti-rabbit IgG 223 AR TIC LE IN PR ES S and cell sections were imaged using a confocal (LSM980; Carl Zeiss, Germany), an automated 225 fluorescence (FV4000; Olympus, Japan), or an inverted fluorescence (IX83; Olympus, Japan) 226 microscope.

    other:

    Article Title: The AKT2/SIRT5/TFEB pathway as a potential therapeutic target in non-neovascular AMD
    Article Snippet: The primary antibodies AKT2 (3063S), p-AKT2 (8599S), SIRT5 (8782S), CTSD (69854S), ULK1 (8054T), FKBP51 (8245S), p-TFEB (37681S), and GFP (2555S) were purchased from Cell Signaling Technology.

    Article Title: Endothelial LRRC8C associates with LRRC8A and LRRC8B to regulate vascular reactivity and blood pressure
    Article Snippet: All other primary antibodies were purchased from Cell Signaling (Danvers, MA): Akt1 (#2938), Akt2 (#3063), p-Akt1 (#4060), p-Akt2 (#8599), eNOS (#32027), p-eNOS (#9571), anti-,8-actin (#8457).



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    The TIM3/GAL9 autocrine stimulatory loop is involved in ALL. (A) GAL9 expression was upregulated in both ALL bone marrow samples and ALL cell lines. (B) TARGET data showed that <t>AKT2,</t> ERK, and NF-κB were activated in ALL. (C) Representative Western blots of total AKT2, total ERK, NF-κB, and GAPDH in Jurkat and Nalm-6 cells transduced with shNC, shLnc-TIM3#1, or shLnc-TIM3#2. Data were shown as mean ± SD and one-way ANOVA was used for multiple-group comparison. **, P<0.01; ****, P<0.0001. ALL, acute lymphoblastic leukemia; ANOVA, analysis of variance; NC, negative-control; SD, standard deviation.
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    The TIM3/GAL9 autocrine stimulatory loop is involved in ALL. (A) GAL9 expression was upregulated in both ALL bone marrow samples and ALL cell lines. (B) TARGET data showed that <t>AKT2,</t> ERK, and NF-κB were activated in ALL. (C) Representative Western blots of total AKT2, total ERK, NF-κB, and GAPDH in Jurkat and Nalm-6 cells transduced with shNC, shLnc-TIM3#1, or shLnc-TIM3#2. Data were shown as mean ± SD and one-way ANOVA was used for multiple-group comparison. **, P<0.01; ****, P<0.0001. ALL, acute lymphoblastic leukemia; ANOVA, analysis of variance; NC, negative-control; SD, standard deviation.
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    Image Search Results


    The TIM3/GAL9 autocrine stimulatory loop is involved in ALL. (A) GAL9 expression was upregulated in both ALL bone marrow samples and ALL cell lines. (B) TARGET data showed that AKT2, ERK, and NF-κB were activated in ALL. (C) Representative Western blots of total AKT2, total ERK, NF-κB, and GAPDH in Jurkat and Nalm-6 cells transduced with shNC, shLnc-TIM3#1, or shLnc-TIM3#2. Data were shown as mean ± SD and one-way ANOVA was used for multiple-group comparison. **, P<0.01; ****, P<0.0001. ALL, acute lymphoblastic leukemia; ANOVA, analysis of variance; NC, negative-control; SD, standard deviation.

    Journal: Translational Cancer Research

    Article Title: Lnc-TIM3 promotes acute lymphoblastic leukemia progression through miR-5094-mediated regulation of TIM3

    doi: 10.21037/tcr-2026-1285

    Figure Lengend Snippet: The TIM3/GAL9 autocrine stimulatory loop is involved in ALL. (A) GAL9 expression was upregulated in both ALL bone marrow samples and ALL cell lines. (B) TARGET data showed that AKT2, ERK, and NF-κB were activated in ALL. (C) Representative Western blots of total AKT2, total ERK, NF-κB, and GAPDH in Jurkat and Nalm-6 cells transduced with shNC, shLnc-TIM3#1, or shLnc-TIM3#2. Data were shown as mean ± SD and one-way ANOVA was used for multiple-group comparison. **, P<0.01; ****, P<0.0001. ALL, acute lymphoblastic leukemia; ANOVA, analysis of variance; NC, negative-control; SD, standard deviation.

    Article Snippet: Primary antibodies against AKT2, TIM3, ERK, NF-κB, p-AKT2, and p-ERK (CST; 1:1,000), and GAPDH (CST; 1:2,000) were diluted in 5% bovine serum albumin (BSA) (Progenics, China).

    Techniques: Expressing, Western Blot, Transduction, Comparison, Analysis, Negative Control, Standard Deviation